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        New Message Board Archives >> 2004 Posts >> sterile containers
        
(Message started by: mmcadam on Jan 18^th, 2004, 9:49pm)

Title: sterile containers
Post by mmcadam on Jan 18^th, 2004, 9:49pm

Is there some sort of container that already comes sterile? This boiling and glassware is a real pain I phantom. Is there an easy way without risk of contamination?

Title: Re: sterile containers
Post by don on Jan 18^th, 2004, 10:13pm

WTF??????

Title: Re: sterile containers
Post by kissmyglass on Jan 18^th, 2004, 10:20pm

Don, shroom growing I would imagine....

Title: Re: sterile containers
Post by Mark C on Jan 19^th, 2004, 4:28am

Yes! (http://mycobag.com/)

8)

Title: Re: sterile containers
Post by grumpett on Jan 19^th, 2004, 8:02am

dont know of any containers already sterile but, You can put a bit of water in them and zap them in the microwave for 1 minute and they are supposed to be sterile.
Hope that helps.

Title: Re: sterile containers
Post by Giovanni on Jan 19^th, 2004, 8:52am

I assume your talking about sterile containers for growing mushies? You really do need a pressure cooker to sterilize the jars as they reach only 212 degrees by boiling in water versus 250 degrees @15 pounds of pressure for 45 minutes in a pressure cooker. At 212 degrees, some of the bacteria and molds can still survive. At 250 everything is supposedly dead.

;;D

Title: Re: sterile containers
Post by don on Jan 19^th, 2004, 11:10am

OOOHHHH! LMMFAO.

Thought I logged on to a tupperware support site. LMAO

Title: Re: sterile containers
Post by echo on Jan 19^th, 2004, 11:26am

Can you go the other way?

At -456 all atomic activity stops. (I think my wife emitts that temperature on a regular basis)

Title: Re: sterile containers
Post by floridian on Jan 19^th, 2004, 12:11pm

I haven't tried this method, but have worked as a microbiologist, and it seems promising.

www.mycomasters.com

It involves the use of hydrogen peroxide to sterlize media instead of a steam pressure cooker. Seems to be cheaper than steam as well. The author says he has protocols for sterilizing agar media, grain media, and sawdust. Alot of the information is free on the website, but the author doesn't spell everything out (he's trying to sell books, after all). Worth a look.

Sterile = the complete absence of life. Requires 252 degrees F heat (steam under pressure), chemical, or ionizing radiation.
Santize = the reduction of microbial life forms to acceptable levels.

For pure culture work, sterilization is generally necessary. For cultivation of shiitake and other mushrooms on logs, compost, or woodchips, santization may be adequate, but the risk of failure is higher.